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Fabrication and characterization of Engineered M2-EXO. (A) Flowchart of the fabrication of engineered M2-EXO. (B) Nonengineered M2 exosome and engineered miR-N20 exosome morphology and diameter distribution, as detected by TEM. (C) <t>Nanoparticle</t> tracking analysis (NTA) of the range of particle size distribution of Nonengineered M2 exosome and engineered miR-493-5p exosome. (D, E) Exosome internalization by HUVECs and RAW264.7 cells. (F) Exosome surface markers detected by western blotting. (G) qRT-PCR analysis of miR-493-5p content in exosomes. (H) qRT-PCR analysis of miR-493-5p content in RAW264.7 cells under EXO or EXO@miR-493-5p treatment. Data are presented as mean ± SD and statistical significance was analyzed via Student's two-sided t -test. ∗P value < 0.05; ∗∗P value < 0.01; ∗∗∗P value < 0.001 per; ∗∗∗∗P value < 0.0001 per group by unpaired t -test.
Nanoparticle Tracking Analysis, supplied by Malvern Panalytical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fabrication and characterization of Engineered M2-EXO. (A) Flowchart of the fabrication of engineered M2-EXO. (B) Nonengineered M2 exosome and engineered miR-N20 exosome morphology and diameter distribution, as detected by TEM. (C) <t>Nanoparticle</t> tracking analysis (NTA) of the range of particle size distribution of Nonengineered M2 exosome and engineered miR-493-5p exosome. (D, E) Exosome internalization by HUVECs and RAW264.7 cells. (F) Exosome surface markers detected by western blotting. (G) qRT-PCR analysis of miR-493-5p content in exosomes. (H) qRT-PCR analysis of miR-493-5p content in RAW264.7 cells under EXO or EXO@miR-493-5p treatment. Data are presented as mean ± SD and statistical significance was analyzed via Student's two-sided t -test. ∗P value < 0.05; ∗∗P value < 0.01; ∗∗∗P value < 0.001 per; ∗∗∗∗P value < 0.0001 per group by unpaired t -test.
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Fabrication and characterization of Engineered M2-EXO. (A) Flowchart of the fabrication of engineered M2-EXO. (B) Nonengineered M2 exosome and engineered miR-N20 exosome morphology and diameter distribution, as detected by TEM. (C) <t>Nanoparticle</t> tracking analysis (NTA) of the range of particle size distribution of Nonengineered M2 exosome and engineered miR-493-5p exosome. (D, E) Exosome internalization by HUVECs and RAW264.7 cells. (F) Exosome surface markers detected by western blotting. (G) qRT-PCR analysis of miR-493-5p content in exosomes. (H) qRT-PCR analysis of miR-493-5p content in RAW264.7 cells under EXO or EXO@miR-493-5p treatment. Data are presented as mean ± SD and statistical significance was analyzed via Student's two-sided t -test. ∗P value < 0.05; ∗∗P value < 0.01; ∗∗∗P value < 0.001 per; ∗∗∗∗P value < 0.0001 per group by unpaired t -test.
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Fabrication and characterization of Engineered M2-EXO. (A) Flowchart of the fabrication of engineered M2-EXO. (B) Nonengineered M2 exosome and engineered miR-N20 exosome morphology and diameter distribution, as detected by TEM. (C) <t>Nanoparticle</t> tracking analysis (NTA) of the range of particle size distribution of Nonengineered M2 exosome and engineered miR-493-5p exosome. (D, E) Exosome internalization by HUVECs and RAW264.7 cells. (F) Exosome surface markers detected by western blotting. (G) qRT-PCR analysis of miR-493-5p content in exosomes. (H) qRT-PCR analysis of miR-493-5p content in RAW264.7 cells under EXO or EXO@miR-493-5p treatment. Data are presented as mean ± SD and statistical significance was analyzed via Student's two-sided t -test. ∗P value < 0.05; ∗∗P value < 0.01; ∗∗∗P value < 0.001 per; ∗∗∗∗P value < 0.0001 per group by unpaired t -test.
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Fabrication and characterization of Engineered M2-EXO. (A) Flowchart of the fabrication of engineered M2-EXO. (B) Nonengineered M2 exosome and engineered miR-N20 exosome morphology and diameter distribution, as detected by TEM. (C) <t>Nanoparticle</t> tracking analysis (NTA) of the range of particle size distribution of Nonengineered M2 exosome and engineered miR-493-5p exosome. (D, E) Exosome internalization by HUVECs and RAW264.7 cells. (F) Exosome surface markers detected by western blotting. (G) qRT-PCR analysis of miR-493-5p content in exosomes. (H) qRT-PCR analysis of miR-493-5p content in RAW264.7 cells under EXO or EXO@miR-493-5p treatment. Data are presented as mean ± SD and statistical significance was analyzed via Student's two-sided t -test. ∗P value < 0.05; ∗∗P value < 0.01; ∗∗∗P value < 0.001 per; ∗∗∗∗P value < 0.0001 per group by unpaired t -test.
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nanoparticle tracking analysis nta  (Malvern Panalytical)
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Fabrication and characterization of Engineered M2-EXO. (A) Flowchart of the fabrication of engineered M2-EXO. (B) Nonengineered M2 exosome and engineered miR-N20 exosome morphology and diameter distribution, as detected by TEM. (C) <t>Nanoparticle</t> tracking analysis (NTA) of the range of particle size distribution of Nonengineered M2 exosome and engineered miR-493-5p exosome. (D, E) Exosome internalization by HUVECs and RAW264.7 cells. (F) Exosome surface markers detected by western blotting. (G) qRT-PCR analysis of miR-493-5p content in exosomes. (H) qRT-PCR analysis of miR-493-5p content in RAW264.7 cells under EXO or EXO@miR-493-5p treatment. Data are presented as mean ± SD and statistical significance was analyzed via Student's two-sided t -test. ∗P value < 0.05; ∗∗P value < 0.01; ∗∗∗P value < 0.001 per; ∗∗∗∗P value < 0.0001 per group by unpaired t -test.
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Fabrication and characterization of Engineered M2-EXO. (A) Flowchart of the fabrication of engineered M2-EXO. (B) Nonengineered M2 exosome and engineered miR-N20 exosome morphology and diameter distribution, as detected by TEM. (C) <t>Nanoparticle</t> tracking analysis (NTA) of the range of particle size distribution of Nonengineered M2 exosome and engineered miR-493-5p exosome. (D, E) Exosome internalization by HUVECs and RAW264.7 cells. (F) Exosome surface markers detected by western blotting. (G) qRT-PCR analysis of miR-493-5p content in exosomes. (H) qRT-PCR analysis of miR-493-5p content in RAW264.7 cells under EXO or EXO@miR-493-5p treatment. Data are presented as mean ± SD and statistical significance was analyzed via Student's two-sided t -test. ∗P value < 0.05; ∗∗P value < 0.01; ∗∗∗P value < 0.001 per; ∗∗∗∗P value < 0.0001 per group by unpaired t -test.
Ns300 Nanoparticle Tracking Analyzer, supplied by Malvern Panalytical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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Fabrication and characterization of Engineered M2-EXO. (A) Flowchart of the fabrication of engineered M2-EXO. (B) Nonengineered M2 exosome and engineered miR-N20 exosome morphology and diameter distribution, as detected by TEM. (C) Nanoparticle tracking analysis (NTA) of the range of particle size distribution of Nonengineered M2 exosome and engineered miR-493-5p exosome. (D, E) Exosome internalization by HUVECs and RAW264.7 cells. (F) Exosome surface markers detected by western blotting. (G) qRT-PCR analysis of miR-493-5p content in exosomes. (H) qRT-PCR analysis of miR-493-5p content in RAW264.7 cells under EXO or EXO@miR-493-5p treatment. Data are presented as mean ± SD and statistical significance was analyzed via Student's two-sided t -test. ∗P value < 0.05; ∗∗P value < 0.01; ∗∗∗P value < 0.001 per; ∗∗∗∗P value < 0.0001 per group by unpaired t -test.

Journal: Materials Today Bio

Article Title: MicroRNA-493-5p engineered exosomes delivered via piezoelectric microneedles for epigenetic modulation of macrophages in diabetic wound healing

doi: 10.1016/j.mtbio.2026.102931

Figure Lengend Snippet: Fabrication and characterization of Engineered M2-EXO. (A) Flowchart of the fabrication of engineered M2-EXO. (B) Nonengineered M2 exosome and engineered miR-N20 exosome morphology and diameter distribution, as detected by TEM. (C) Nanoparticle tracking analysis (NTA) of the range of particle size distribution of Nonengineered M2 exosome and engineered miR-493-5p exosome. (D, E) Exosome internalization by HUVECs and RAW264.7 cells. (F) Exosome surface markers detected by western blotting. (G) qRT-PCR analysis of miR-493-5p content in exosomes. (H) qRT-PCR analysis of miR-493-5p content in RAW264.7 cells under EXO or EXO@miR-493-5p treatment. Data are presented as mean ± SD and statistical significance was analyzed via Student's two-sided t -test. ∗P value < 0.05; ∗∗P value < 0.01; ∗∗∗P value < 0.001 per; ∗∗∗∗P value < 0.0001 per group by unpaired t -test.

Article Snippet: Scanning electron microscopy (SEM, Zeiss Merlin) and laser confocal microscopy (Leica TCS SP8) were utilized for structural characterization, while nanoparticle tracking analysis (NTA, Malvern NanoSight NS300) quantified exosome size distribution.

Techniques: Western Blot, Quantitative RT-PCR